血管活性腸肽(VIP)重組蛋白
Recombinant Vasoactive Intestinal Peptide (VIP)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
- 編號RPA380Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量16.0kDa
- 實際分子量18kDa(差異分析請參閱說明書)
- 片段與標簽Leu28~Gly159 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點7.1
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 1056¥ 2640¥ 5280¥ 15840¥ 39600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
SDS-PAGE圖
通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA380Mu01 | 血管活性腸肽(VIP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA380Mu02 | 血管活性腸肽(VIP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA380Mu02 | 血管活性腸肽(VIP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA380Mu01 | 血管活性腸肽(VIP)多克隆抗體 | WB; IHC; ICC; IP. |
| CEA380Mu | 血管活性腸肽(VIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA380Mu | 血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA380Mu11 | 血管活性腸肽(VIP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Clinical Genetics | VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population[Wiley: source] |
| Human immunology | Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy[Pubmed: 23800435] |
| Headache: The Journal of Head and Face Pain | CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine[Onlinelibrary: head.12372] |
| World J Gastroenterol | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide[NCBI: PMC4000510] |
| Cephalalgia? | Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine[Pubmed:24847167] |
| J Huazhong Univ Sci Technolog Med Sci. | Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients[Pubmed:25318892] |
| PLOS ONE | Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters[Pubmed:25647228] |
| PLOS ONE | Aqueous Fraction of[PubMed: 25647228] |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation[PubMed: 25836921] |
| World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones[Pubmed:27350732] |
| Annals of Allergy, Asthma and Immunology | Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis[Pubmed:27156748] |
| Cephalalgia. | Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients[Pubmed:27250233] |
| Headache | No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine[pubmed:27634731] |
| Sci Rep. | Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.[pubmed:28054652] |
| BMC Complementary and Alternative Medicine | [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice[10.1186/s12906-017-1903-0] |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation[10.1007/s13765-017-0327-3] |
| Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis[Pubmed:28914594] |
| Evidence-Based?Complementary and Alternative Medicine | Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids[Pubmed:29853946] |
| Revista do Instituto de Medicina Tropical de S?o Paulo | Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients[Pubmed: 30365640] |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement[Pubmed: 30562932] |
| Science?of?Food?and?Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer[Pubmed: 30479041] |
| MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice[Pubmed: 32951099] |
| Journal of Cystic Fibrosis | Disrupted local innervation results in less VIP expression in CF mice tissues[Pubmed: 32600901] |
| Biomedical Journal of Scientific & Technical Research | Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?[] |
| Journal of tranditional chinese medical sciences | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome[] |
| J Cyst Fibros | VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD)[34034984] |
| Neurol Sci | Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study[34698942] |
| Endocr J | A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience[Pubmed:35644576] |