血管活性腸肽(VIP)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Vasoactive Intestinal Peptide (VIP)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
- 編號CEA380Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍6.17-500pg/mL
- 靈敏度最小可檢測劑量小于等于2.71pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2722¥ 3888¥ 17496¥ 33048¥ 272160
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測血管活性腸肽(VIP)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管活性腸肽(VIP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-96 | 88 |
| EDTA plasma(n=5) | 79-95 | 90 |
| heparin plasma(n=5) | 85-97 | 90 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的血管活性腸肽(VIP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管活性腸肽(VIP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 88-102% | 91-105% | 78-104% |
| EDTA plasma(n=5) | 78-101% | 80-93% | 90-97% | 89-101% |
| heparin plasma(n=5) | 97-104% | 78-89% | 99-105% | 99-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數。
實驗原理
本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將血管活性腸肽(VIP)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物，然后加入HRP標記的親和素，經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA380Mu01 | 血管活性腸肽(VIP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA380Mu02 | 血管活性腸肽(VIP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA380Mu02 | 血管活性腸肽(VIP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA380Mu01 | 血管活性腸肽(VIP)多克隆抗體 | WB; IHC; ICC; IP. |
| CEA380Mu | 血管活性腸肽(VIP)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA380Mu | 血管活性腸肽(VIP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA380Mu11 | 血管活性腸肽(VIP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Clinical Genetics | VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population[Wiley: source] |
| Human immunology | Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy[Pubmed: 23800435] |
| Headache: The Journal of Head and Face Pain | CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine[Onlinelibrary: head.12372] |
| World J Gastroenterol | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide[NCBI: PMC4000510] |
| Cephalalgia? | Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine[Pubmed:24847167] |
| J Huazhong Univ Sci Technolog Med Sci. | Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients[Pubmed:25318892] |
| PLOS ONE | Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters[Pubmed:25647228] |
| PLOS ONE | Aqueous Fraction of[PubMed: 25647228] |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation[PubMed: 25836921] |
| World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones[Pubmed:27350732] |
| Annals of Allergy, Asthma and Immunology | Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis[Pubmed:27156748] |
| Cephalalgia. | Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients[Pubmed:27250233] |
| Headache | No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine[pubmed:27634731] |
| Sci Rep. | Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.[pubmed:28054652] |
| BMC Complementary and Alternative Medicine | [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice[10.1186/s12906-017-1903-0] |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation[10.1007/s13765-017-0327-3] |
| Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis[Pubmed:28914594] |
| Evidence-Based?Complementary and Alternative Medicine | Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids[Pubmed:29853946] |
| Revista do Instituto de Medicina Tropical de S?o Paulo | Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients[Pubmed: 30365640] |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement[Pubmed: 30562932] |
| Science?of?Food?and?Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer[Pubmed: 30479041] |
| MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice[Pubmed: 32951099] |
| Journal of Cystic Fibrosis | Disrupted local innervation results in less VIP expression in CF mice tissues[Pubmed: 32600901] |
| Biomedical Journal of Scientific & Technical Research | Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?[] |
| Journal of tranditional chinese medical sciences | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome[] |
| J Cyst Fibros | VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD)[34034984] |
| Neurol Sci | Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study[34698942] |
| Endocr J | A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience[Pubmed:35644576] |
