無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白
Recombinant Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- 編號RPP155Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌, 細胞外基質(zhì)
- 預(yù)測分子量41.0kDa
- 實際分子量41kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Met1~Phe297 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點7.8
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPP155Ra01 | 無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP155Ra01 | 無翅型MMTV整合位點家族成員3A(WNT3A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAP155Ra21 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | WB; IHC; ICC; IP. |
| SEP155Ra | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEP155Ra | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP155Ra | 無翅型MMTV整合位點家族成員3A(WNT3A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSP155Ra01 | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling[Pubmed:25801232] |
| J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs[PubMed: 26209081] |
| PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification[pubmed:28380024] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle[Pubmed:30151071] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer[Doi: 10.15369/sujms.30.359] |
| Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma[Pubmed: 31737122] |
| CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection[Pubmed: 32920199] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885] |