基質(zhì)金屬蛋白酶1(MMP1)重組蛋白
Recombinant Matrix Metalloproteinase 1 (MMP1)
CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
- 編號RPA097Ov01
- 物種Ovis aries; Ovine (Sheep，綿羊)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位細(xì)胞外基質(zhì)
- 預(yù)測分子量55.1kDa
- 實際分子量58kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Phe19~Asn467 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點6.4
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 984¥ 2460¥ 4920¥ 14760¥ 36900
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Ovis aries; Ovine (Sheep，綿羊) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA097Ov01 | 基質(zhì)金屬蛋白酶1(MMP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA097Ov01 | 基質(zhì)金屬蛋白酶1(MMP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA097Ov01 | 基質(zhì)金屬蛋白酶1(MMP1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA097Ov21 | 基質(zhì)金屬蛋白酶1(MMP1)單克隆抗體 | WB; IHC; ICC; IP. |
| SCA097Ov | 基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA097Ov | 基質(zhì)金屬蛋白酶1(MMP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Parasitology Research | Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice[SpringerLink: j33n80517577535k] |
| Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier[Pubmed: 23649093] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] |
| The Journal of Pathology | Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation[Pubmed:25186281] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| J Immunol | Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis[PubMed: 26268658] |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes[PubMed: 25938977] |
| J Periodontal Implant Sci | Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol[pmc:PMC4848383] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| Cellular?& Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes[pubmed:25938977] |
| Molecular Medicine Reports | Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells[pubmed:28447732] |
| Analytical Methods | The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique[c6ay01856d] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Analytical Methods | SPRI biosensors for quantitative determination of matrix metalloproteinase-2[DOI:10.1039/C7AY00786H] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| European?Heart?Journal | Biomarker‐based phenotyping of myocardial fibrosis identifies patients with heart failure with preserved ejection fraction resistant to the beneficial effects of?…[Pubmed:29709099] |
| Journal of Agricultural and?Food?Chemistry | Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice[Pubmed:30032605] |
| Oral?Diseases? | TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis[Pubmed:29989318] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis[10.1080:09712119.2017.1335208] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis[Pubmed: 30939166] |
| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ[] | |
| International Journal of Molecular Sciences | A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor[Pubmed: 31052424] |
| INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | MT 1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation[] |
| Journal of Plastic, Reconstructive & Aesthetic Surgery | Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage[Pubmed: 31734236] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis[Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] |
| JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling?…[Pubmed: 32745509] |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice[33890588] |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK?…[Pubmed:35660352] |
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes[Pubmed:35340587] |