Ⅰ型膠原α1(COL1a1)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Collagen Type I Alpha 1 (COL1a1)
COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
- 編號SEA350Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.058ng/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測Ⅰ型膠原α1(COL1a1)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型膠原α1(COL1a1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-97 | 93 |
| EDTA plasma(n=5) | 95-103 | 99 |
| heparin plasma(n=5) | 94-102 | 99 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的Ⅰ型膠原α1(COL1a1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型膠原α1(COL1a1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 93-102% | 78-96% | 92-105% |
| EDTA plasma(n=5) | 84-102% | 96-104% | 96-104% | 99-105% |
| heparin plasma(n=5) | 81-93% | 93-102% | 83-103% | 90-104% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將Ⅰ型膠原α1(COL1a1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的Ⅰ型膠原α1(COL1a1)與連接于固相載體上的抗體結合，然后加入生物素化的Ⅰ型膠原α1(COL1a1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的Ⅰ型膠原α1(COL1a1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA350Ra01 | Ⅰ型膠原α1(COL1a1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA350Ra01 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA350Ra21 | Ⅰ型膠原α1(COL1a1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA350Ra | Ⅰ型膠原α1(COL1a1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA350Ra | Ⅰ型膠原α1(COL1a1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology | Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study[ScienceDirect: S1079210410004919] |
| Journal of Biomedical Materials | Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study[Wiley: source] |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats[Pubmed: 24077847] |
| J Voice. | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study [Pubmed: 24495429] |
| Arch Oral Biol. | Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells.[Pubmed: 24370188] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria[Pubmed:25545625] |
| UNIVERSA MEDICINA | Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture[Univmed:Source] |
| International Journal of?Neuropsychopharmacology | The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study.[pubmed:27477355] |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396] |
| Nephron. | Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor[pubmed:28768285] |
| Cell?and?Tissue?Research | Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and?…[Pubmed:29637306] |
| International Journal of Molecular?Medicine | LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway[Pubmed: 30320382] |
| International Journal of Molecular?Sciences | Association between Plasma HMGB-1 and Silicosis: A Case-Control Study[Pubmed: 30558126] |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection[Pubmed: 31706945] |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm[] |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response[] |
| Scientific reports | Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells[33911160] |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model[Pubmed:35682677] |
