S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)
MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
- 編號SEB792Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍7.8-500pg/mL
- 靈敏度最小可檢測劑量小于等于3.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
- 欲了解實(shí)際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測S100鈣結(jié)合蛋白A8(S100A8)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的S100鈣結(jié)合蛋白A8(S100A8)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 78-98 | 95 |
| heparin plasma(n=5) | 91-99 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的S100鈣結(jié)合蛋白A8(S100A8)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中S100鈣結(jié)合蛋白A8(S100A8)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 83-93% | 89-97% | 98-105% |
| EDTA plasma(n=5) | 79-98% | 78-101% | 94-101% | 79-101% |
| heparin plasma(n=5) | 89-101% | 97-104% | 83-99% | 79-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將S100鈣結(jié)合蛋白A8(S100A8)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的S100鈣結(jié)合蛋白A8(S100A8)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的S100鈣結(jié)合蛋白A8(S100A8)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的S100鈣結(jié)合蛋白A8(S100A8)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC |
| PAB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB792Mu81 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB792Mu71 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB792Mu21 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB792Mu | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB792Mu | S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice[ScienceDirect: S0006291X1200352X] |
| American Journal of Reproductive Immunology | Association of Increased S100A8 Serum Protein with Early Pregnancy Loss[Pubmed:25252120] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation[PubMed: 25934702] |
| J Immunol | S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet[PubMed: 26608915] |
| PLoS?One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation[Pubmed:26889680] |
| Journal?of?Alzheimer's?Disease | Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology.[pubmed:27911324] |
| ?Journal of Functional Foods | Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation[S1756464617300208] |
| International?Journal?of?Molecular?Medicine | Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells[pubmed:28498464] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds.[pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis[10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis[10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation[Pubmed:29496993] |
| European?review for?medical?and pharmacological?sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα.[Pubmed:29949169] |
| Foodborne Pathogens and Disease | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model[Pubmed: 31116394] |
| Kidney International Reports | Systemic Inflammation Precedes Microalbuminuria in Diabetes[] |
| Cancer Management and Research | iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia[Pubmed: 31440093] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study[Pubmed: 31856598] |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis[] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays[34012227] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in[] |
