衰老關鍵蛋白3(FBLN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Fibulin 3 (FBLN3)
EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5
產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測衰老關鍵蛋白3(FBLN3)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的衰老關鍵蛋白3(FBLN3)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 89-101 | 93 |
| heparin plasma(n=5) | 96-104 | 99 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的衰老關鍵蛋白3(FBLN3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中衰老關鍵蛋白3(FBLN3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 86-104% | 78-94% | 91-99% |
| EDTA plasma(n=5) | 91-98% | 78-94% | 87-94% | 90-98% |
| heparin plasma(n=5) | 92-99% | 98-105% | 89-96% | 96-103% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將衰老關鍵蛋白3(FBLN3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的衰老關鍵蛋白3(FBLN3)與連接于固相載體上的抗體結合，然后加入生物素化的衰老關鍵蛋白3(FBLN3)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的衰老關鍵蛋白3(FBLN3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPF422Mu01 | 衰老關鍵蛋白3(FBLN3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF422Mu02 | 衰老關鍵蛋白3(FBLN3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF422Mu01 | 衰老關鍵蛋白3(FBLN3)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF422Mu02 | 衰老關鍵蛋白3(FBLN3)多克隆抗體 | WB; IHC; ICC; IP. |
| SEF422Mu | 衰老關鍵蛋白3(FBLN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF422Mu | 衰老關鍵蛋白3(FBLN3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Anticancer Res. | YKL-40 and mesothelin in the blood of patients with malignant mesothelioma, lung cancer and asbestosis.[Pubmed: 24324091] |
| Oncology Reports | Role of fibulin-3 in lung cancer: In vivo and in vitro analyses[Spandidos-publications: Source] |
| Current Pulmonology Reports | Discovery of new biomarkers for malignant mesothelioma[Springer:Source] |
| United States Patent Application 20140378336 | DIAGNOSIS OF CELL PROLIFERATIVE DISEASES[Freepatentsonline:Source] |
| Thorax. | Comparison of fibulin-3 and mesothelin as markers in malignant mesothelioma[Pubmed:Pmc4174124] |
| Egyptian Journal of Chest Diseases and Tuberculosis | Value of plasma and pleural effusion fibulin-3 levels in the diagnosis of malignant pleural mesothelioma effusions[Pubmed:23050525] |
| Radiol Oncol | Fibulin-3 as a biomarker of response to treatment in malignant mesothelioma[PubMed: 26401134] |
| British Journal of Cancer | Fibulin-3 levels in malignant pleural mesothelioma are associated with prognosis but not diagnosis[PubMed: 26263483] |
| J Thorac Oncol. | Plasma Biomarker Enrichment of Clinical Prognostic Indices in Malignant Pleural Mesothelioma.[Pubmed:26903362] |
| BMJ Open | Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational study [pubmed:27884852] |
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| BMJ Journals | Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational …[e013324.abstract] |
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| The Knee | Association of fibulin-3 concentrations with the presence and severity of knee osteoarthritis: A cross-sectional study[pubmed:29195846] |
| Disease markers | Plasma Fibulin-3 as a Potential Biomarker for Patients with Asbestos-Related Diseases in the Han Population[pubmed:29200597] |
| Molecular?Medicine?Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes[Pubmed:29749501] |
| Oncotarget | The matrix protein Fibulin-3 promotes KISS1R induced triple negative breast cancer cell invasion[Pubmed:30046386] |
| Advances in?Clinical?and Experimental?Medicine | Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with?…[68362.pdf] |
| PLoS One | Prediction of pneumoconiosis by serum and urinary biomarkers in workers exposed to asbestos-contaminated minerals[Pubmed: 30946771] |
