玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Vitronectin (VTN)
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
- 編號KSB041Ra01
 - 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
 - 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
 - 可檢測樣本Plasma.
 - 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
 - 可檢測范圍7.81-500ng/mL
 - 可適靈敏度3.7ng/mL
 - 應用Main materials for "Do It (ELISA Kit) Yourself".
 - 下載英文說明書 中文說明書
 - 規(guī)格96T*596T*1096T*2096T*5096T*100
 - 價格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
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特異性
試劑盒中的抗體對玻連蛋白(VTN)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) | 
| RPB041Ra01 | 玻連蛋白(VTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| APB041Ra01 | 玻連蛋白(VTN)活性蛋白 | Cell?culture;?Activity?Assays. | 
| PAB041Ra01 | 玻連蛋白(VTN)多克隆抗體 | WB; IHC; ICC; IP. | 
| MAB041Ra21 | 玻連蛋白(VTN)單克隆抗體 | WB; IHC; ICC; IP. | 
| SEB041Ra | 玻連蛋白(VTN)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| LMB041Ra | 玻連蛋白(VTN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| KSB041Ra01 | 玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". | 
參考文獻
| 雜志 | 參考文獻 | 
| Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563] | 
| PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance[PubMed: PMC3754973] | 
| Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE[Pubmed: 23944848] | 
| Analytical Biochemistry | A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots[Pubmed:29154788] | 
| Journal of Materials Chemistry B | Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs[Doi: 10.1039/C8TB02283F?] | 
| JOURNAL OF NEPHROLOGY | Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts[33275196] | 


