多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Syndecan 1 (SDC1)
CD138; SDC; SYND1; Syndecan Proteoglycan 1
- 編號(hào)KSB966Hu01
- 物種Homo sapiens (Human，人)相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.56-100ng/mL
- 可適靈敏度0.63ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)多配體蛋白聚糖1(SDC1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPB966Hu62 | 多配體蛋白聚糖1(SDC1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu01 | 多配體蛋白聚糖1(SDC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB966Hu61 | 多配體蛋白聚糖1(SDC1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu02 | 多配體蛋白聚糖1(SDC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu03 | 多配體蛋白聚糖1(SDC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB966Hu01 | 多配體蛋白聚糖1(SDC1)多克隆抗體 | WB; IHC; ICC/IF |
| FAB966Hu01 | 抗多配體蛋白聚糖1(SDC1)多克隆抗體 | Flow cytometry. |
| FAB966Hu41 | 抗多配體蛋白聚糖1(SDC1)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| FAB966Hu51 | 抗多配體蛋白聚糖1(SDC1)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
| FAB966Hu81 | 抗多配體蛋白聚糖1(SDC1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| MAB966Hu21 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB; IHC |
| MAB966Hu23 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB |
| MAB966Hu22 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB |
| MAB966Hu24 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB |
| MAB966Hu25 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB |
| RAB966Hu21 | 多配體蛋白聚糖1(SDC1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB966Hu27 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | IHC |
| MAB966Hu29 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB; IHC; ICC/IF |
| SEB966Hu | 多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB966Hu | 多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB966Hu | 多配體蛋白聚糖1(SDC1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB966Hu01 | 多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular and Cellular Biochemistry | Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction[SpringerLink: a201w3l18k257462] |
| Inflammation | Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats[Springer: Source] |
| Atherosclerosis. | Damage of the endothelial glycocalyx in chronic kidney disease[Pubmed:24727235] |
| Journal of Trauma and Acute Care Surgery | Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury[Pubmed:25058256] |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance[Pubmed:25692257] |
| Mediators Inflamm | Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding[PubMed: 26199464] |
| Transplantation | Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.[PubMed: 26371596] |
| Clinical and Experimental Nephrology | Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury[pubmed:27405618] |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection[pubmed:27643669] |
| Danish National RESEARCH DATABASE | Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats[10.1089] |
| Journal of Neurotrauma | Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.[doi/10.1089/neu.2016.4574] |
| British Journal of Anaesthesia | Volume kinetics of Ringer's lactate solution in acute inflammatory disease[10.1016:j.bja.2018.04.023] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology | MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge[Pubmed:29903730] |
| Shock | Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis[Pubmed:28591008] |
| Shock | Experimental models of endotheliopathy: impact of shock severity[Pubmed:28697004] |
| Journal of?Trauma?and?Acute?Care?Surgery | Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat[Pubmed:28930945] |
| The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions[173015_06.pdf] | |
| Journal of Surgical Research | Plasma ameliorates endothelial dysfunction in burn injury[Doi: 10.1016/j.jss.2018.08.027] |
| Journal of Anesthesia | Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model[Pubmed: 30374889] |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass[Pubmed: 30687934] |
| Journal of Cardiothoracic and Vascular Anesthesial | Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit[Pubmed: 31787433] |
| Hepatobiliary & Pancreatic Diseases International | Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP[Pubmed: 31919038] |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study[] | |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis[33375342] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency[34119534] |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?…[34423832] |
| Shock | Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke[34524269] |
| Exp Anim | Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model[Pubmed:35110424] |
| Journal of Emergency Medicine | Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase[] |