線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)
- 編號KSX132Ra11
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍2.47-200ng/mL
- 可適靈敏度0.94ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對線粒體開放閱讀框12S rRNA-C(MOTS-c)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| CEX132Ra | 線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMX132Ra | 線粒體開放閱讀框12S rRNA-C(MOTS-c)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSX132Ra11 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Pediatric?Diabetes? | Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance[Pubmed:29691953] |
| Rapid?Commun?Mass?Spectrom | Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes[Pubmed: 30394592] |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via?…[Pubmed: 32880686] |
| Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats[] | |
| FASEB JOURNAL | Neural cell?\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis[33454965] |
| Biochim Biophys Acta Mol Basis Dis | MOTS-c interacts synergistically with exercise intervention to regulate PGC-1|á expression, attenuate insulin resistance and enhance glucose metabolism in mice via??-[33722744] |
| researchsquare | Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation[] |
| Mol Psychiatry | Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder[34471251] |
| Pharmacol Res | The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus[34798268] |
| Ann Neurol | SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19[Pubmed:35285072] |
| J Clin Med | Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational?…[Pubmed:35683389] |