凝溶膠蛋白(GSN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- 編號KSA372Ra01
 - 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
 - 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
 - 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 - 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
 - 可檢測范圍0.312-20ng/mL
 - 可適靈敏度0.14ng/mL
 - 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
 - 下載英文說明書 中文說明書
 - 規(guī)格96T*596T*1096T*2096T*5096T*100
 - 價格¥ 7045¥ 11742¥ 21136¥ 41097¥ 70452
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特異性
試劑盒中的抗體對凝溶膠蛋白(GS)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) | 
| RPA372Ra01 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| PAA372Ra01 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. | 
| LAA372Ra81 | 凝溶膠蛋白(GSN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. | 
| LAA372Ra71 | 凝溶膠蛋白(GSN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. | 
| SEA372Ra | 凝溶膠蛋白(GSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| LMA372Ra | 凝溶膠蛋白(GSN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| KSA372Ra01 | 凝溶膠蛋白(GSN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". | 
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) | 
| Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy[SpringerLink: 3585r8h46q7r0171] | 
| Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury[BioMed: 17422094] | 
| Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563] | 
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia[Pubmed: 24239294] | 
| The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases[Pubmed: 24565402] | 
| BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study[Pubmed:Pmc4132929] | 
| Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury[Pubmed:25164111] | 
| Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease[PubMed: 25864780] | 
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients[Pubmed:26680509] | 
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients.[pubmed:26680509] | 
| Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.[pubmed:28365030] | 
| Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720] | 
| World?Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children[Pubmed:29427164] | 
| Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various?…[Pubmed:35327525] | 


