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脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4)

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

  • 脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)脂肪細(xì)胞型脂肪酸結(jié)合蛋白(FABP4)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB693Mu02脂肪酸結(jié)合蛋白 4(FABP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB693Mu01脂肪酸結(jié)合蛋白 4(FABP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPB693Mu61脂肪酸結(jié)合蛋白 4(FABP4)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB693Mu01脂肪酸結(jié)合蛋白 4(FABP4)活性蛋白Cell?culture;?Activity?Assays.
APB693Mu61脂肪酸結(jié)合蛋白 4(FABP4)活性蛋白Cell?culture;?Activity?Assays.
PAB693Mu06脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體WB; IHC; ICC; IP.
PAB693Mu02脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體WB; IHC; ICC; IP.
PAB693Mu01脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體WB; IHC; ICC; IP.
LAB693Mu71脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
LAB693Mu81脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體(異硫氰酸熒光素標(biāo)記)WB; IHC; ICC; IF.
SEB693Mu脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB693Mu脂肪酸結(jié)合蛋白 4(FABP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB693Mu01脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志參考文獻(xiàn)
Clinical and Molecular Hepatology Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH[PubMed: 23091808]
journal of Human HypertensionSiesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults[PubMed: 26134622]
genetics and molecular researchOverexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice[PubMed: 25867423]
Food ChemCranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes[PubMed: 25952883]
PlacentaIncreased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia[Pubmed:26992681]
Clin Chim Acta.Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies.[pubmed:28161274]
Oxidative?Medicine?and?Cellular?LongevityChanges of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?…[Pubmed:29849871]
Nutrition &?DiabetesLack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans[Pubmed:29335416]
American Journal of Cancer Research25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4[Pubmed: 31720079]
J Magn Reson ImagingWater Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?…[Pubmed:35212074]
cellsThe Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure[Pubmed:35455943]
Bone ResearchFABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis[Pubmed:35729106]