雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Estrogen Receptor Beta (ERb)
ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2
- 編號KSA437Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.117ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對雌激素受體β(ERb)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA437Hu01 | 雌激素受體β(ERb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA437Hu01 | 雌激素受體β(ERb)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA437Hu71 | 雌激素受體β(ERb)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA437Hu81 | 雌激素受體β(ERb)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| FAA437Hu11 | 抗雌激素受體β(ERb)多克隆抗體(標記) | FCM |
| MAA437Hu22 | 雌激素受體β(ERb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA437Hu21 | 雌激素受體β(ERb)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA437Hu | 雌激素受體β(ERb)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA437Hu | 雌激素受體β(ERb)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA437Hu | 雌激素受體β(ERb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA437Hu01 | 雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling[Pubmed:24846829] |
| Biochem Biophys Res Commun | Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas[PubMed: 26529545] |
| Mol Neurobiol | Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia[PubMed: 26476840] |
| J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling[PubMed: 24846830] |
| Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts[science:S1001074216300924] |
| Biol Open. | Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine.[pubmed:27875242] |
| Biomedicine & Pharmacotherapy | Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY[Pubmed:29807224] |
| Journal of Applied Pharmaceutical Science | Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract[Pbulication:325682424] |
| Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts[] |
| Life Sciences | MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl[34273377] |
| Cardiometry | Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue[] |