細(xì)胞色素C(CYCS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Cytochrome C (CYCS)
Cyt-C; HCS; CYC; CytC
- 編號(hào)KSA594Ra01
 - 物種Rattus norvegicus (Rat,大鼠)相同的名稱(chēng),不同的物種。
 - 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
 - 可檢測(cè)樣本Ttissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 - 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
 - 可檢測(cè)范圍0.312-20ng/mL
 - 可適靈敏度0.14ng/mL
 - 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
 - 規(guī)格96T*596T*1096T*2096T*5096T*100
 - 價(jià)格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
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特異性
試劑盒中的抗體對(duì)細(xì)胞色素C(CYCS)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) | 
| RPA594Ra01 | 細(xì)胞色素C(CYCS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| PAA594Ra01 | 細(xì)胞色素C(CYCS)多克隆抗體 | WB | 
| SEA594Ra | 細(xì)胞色素C(CYCS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| LMA594Ra | 細(xì)胞色素C(CYCS)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| KSA594Ra01 | 細(xì)胞色素C(CYCS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". | 
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) | 
| J Ethnopharmacol | Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening[PubMed: 26320687] | 
| Cell Stress Chaperones | Gypenosides alleviate myocardial ischemia-reperfusion injury via attenuation of oxidative stress and preservation of mitochondrial function in rat heart[Pubmed:26800973] | 
| Molecules | Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway[Pubmed:27248986] | 
| International Journal of Biological Macromolecules | A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats[pubmed:28709897] | 
| West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.[] | 
| PeerJ. | Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway[Pubmed: 30723634] | 
| Biological trace element research | High Concentrations of Boric Acid Trigger Concentration-Dependent Oxidative Stress, Apoptotic Pathways and Morphological Alterations in DU-145 Human Prostate?…[Pubmed: 31066018] | 
| Biological Trace Element Research | The Role of Oxidative Stress, Renal Inflammation, and Apoptosis in Post Ischemic Reperfusion Injury of Kidney Tissue: the Protective Effect of Dose-Dependent Boric?…[] | 
| Cell?Stress Chaperones | Betaine suppresses cell proliferation by increasing oxidative stress–mediated apoptosis and inflammation in DU-145 human prostate cancer cell line[Pubmed: 31368044] | 
| Biological trace element research | Concentration-Dependent Effects of Zinc Sulfate on DU-145 Human Prostate Cancer Cell Line: Oxidative, Apoptotic, Inflammatory, and Morphological Analyzes[Pubmed: 31463762] | 
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway.[Pubmed: 32603817] | 
| J Biochem Mol Toxicol | Concanavalin A induces apoptosis in a dose?\dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells[33604990] | 
| Reprod Sci | Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses[34101148] | 
| Environ Sci Pollut Res Int | Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological??-[34097215] | 
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats[34426252] | 
| Redox Biol | 12/15–Lipoxygenase debilitates mitochondrial health in intermittent hypobaric hypoxia induced neuronal damage: An in vivo study[Pubmed:34979449] | 
| Cancers (Basel) | In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the?…[Pubmed:35326689] | 
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death[Pubmed:35732625] | 
| Journal of Nanobiotechnology | Microbial hydrogen “manufactory” for enhanced gas therapy and self-activated immunotherapy via reduced immune escape[Pubmed:35705974] | 
| Value Health Sci | Oksidatif, Apoptotik ve ?nflamatuar Sinyal Yolaklar? üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif Etkileri[] | 
| Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network[] | 


