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連環(huán)蛋白β1(β-catenin)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Beta Catenin (β-catenin)

CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein

  • 連環(huán)蛋白β1(β-catenin)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 連環(huán)蛋白β1(β-catenin)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對連環(huán)蛋白β1(CTNNb1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

參考文獻(xiàn)

雜志參考文獻(xiàn)
Clinica Chimica ActaVersican and its associated molecules: Potential diagnostic markers for multiple myeloma[Pubmed:25623955]
Journal of Bone and Mineral ResearchAdverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth[Pubmed:25639729]
Am J Transl ResRole of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats[PubMed: 26396666]
Scientific ReportsIs there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA.[pubmed:28533512]
Life SciencesHepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618]
Scientific ReportsIs there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA[Pubmed: 28533512]
The Showa University Journal of Medical SciencesEribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer[Doi: 10.15369/sujms.30.359]
Advances in?Medical?SciencesUrinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney?[Pubmed: 30738287]
Biomed PharmacotherSilence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway[Pubmed: 32028241]
Journal of Cosmetic DermatologyNovel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling?…[Pubmed: 33179848]
NutrientsHydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia[Pubmed: 32784915]
OncotargetAmalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme[33473259]
CytokineQNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model[34564023]