密封蛋白(OCLN)多克隆抗體
Polyclonal Antibody to Occludin (OCLN)
Tight Junction Protein Occludin TM4 Minus
- 編號(hào)PAC228Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPC228Hu01-密封蛋白(OCLN)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.31mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 630¥ 1470¥ 2100¥ 5250¥ 21000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: Human Lung lysate; Lane3: Mouse Liver lysate; Lane4: HepG2 cell lysate Primary Ab: 2μg/ml Rabbit Anti-Human OCLN Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Cerebellum lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human OCLN Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)OCLN的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別OCLN。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC228Hu01 | 密封蛋白(OCLN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC228Hu01 | 密封蛋白(OCLN)多克隆抗體 | WB |
| MAC228Hu22 | 密封蛋白(OCLN)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC228Hu | 密封蛋白(OCLN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC228Hu | 密封蛋白(OCLN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Brain Research | HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9[ScienceDirect: S0006899311021408] |
| Plos one | Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation[PubMed: PMC3688973] |
| Journal of Neurotrauma | A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adults[PubMed: 25794137] |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury[2016:0103140] |
| Sci Rep.? | Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke[articles:srep40331] |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal?…[Pubmed:29375374] |
| Thorax | Fas activation alters tight junction proteins in acute lung injury[Pubmed: 30385692] |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets[Pubmed: 31426309] |
| DISEASE MARKERS | Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumors[Pubmed: 32884584] |
| Burns Open | Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)[] |
| PLoS One | A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and?…[34492054] |
| CNS Neuroscience & Therapeutics | Higher serum occludin after successful reperfusion Is associated with early neurological deterioration[Pubmed:35338575] |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?…[] |