N-酰基鞘氨醇酰胺水解酶1(ASAH1)多克隆抗體
Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)
AC; ASAH; PHP; PHP32; Acid Ceramidase; Acylsphingosine deacylase; Putative 32 kDa heart protein
- 編號PAC302Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPC302Hu01-N-?；拾贝减０匪饷?(ASAH1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ASAH1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant ASAH1, Human.
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Porcine Heart lysate
Primary Ab: 1μg/ml Rabbit Anti-Human ASAH1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對ASAH1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ASAH1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC302Hu01 | N-?；拾贝减０匪饷?(ASAH1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC302Hu01 | N-酰基鞘氨醇酰胺水解酶1(ASAH1)多克隆抗體 | WB; IHC |
| MAC302Hu22 | N-?；拾贝减０匪饷?(ASAH1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC302Hu | N-?；拾贝减０匪饷?(ASAH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC302Hu | N-酰基鞘氨醇酰胺水解酶1(ASAH1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Cytotechnology | The investigation of ceranib-2 on apoptosis and drug interaction with carboplatin in human non small cell lung cancer cells in vitro[pubmed:29230631] |
| Cells | Targeting Acid Ceramidase to Improve the Radiosensitivity of Rectal Cancer[33334013] |