烯醇化酶(ENO3)多克隆抗體
Polyclonal Antibody to Enolase 3 (ENO3)
MSE; Beta Enolase; Enolase 3; 2-phospho-D-glycerate hydro-lyase; Skeletal muscle enolase
- 編號PAB433Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPB433Mu01-烯醇化酶(ENO3)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 616¥ 1436¥ 2052¥ 5130¥ 20520
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Samples: Mouse Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse MSE Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse ENO3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse ENO3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse ENO3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
WB圖
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse MSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse MSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Spleen lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse MSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse MSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse MSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對ENO3的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ENO3。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學：5-20μg/mL;1:25-100
免疫細胞化學：5-20μg/mL;1:25-100
最佳稀釋倍數最終由用戶決定。
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
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相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB433Mu01 | 烯醇化酶(ENO3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB433Mu01 | 烯醇化酶(ENO3)多克隆抗體 | WB; IHC; ICC/IF |
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