三葉因子3(TFF3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Trefoil Factor 3 (TFF3)
HITF; ITF3; TFI; HP1.B; Trefoil Factor 3, Intestinal
- 編號KSB656Hu01
 - 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
 - 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
 - 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 - 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
 - 可檢測范圍62.5-4000pg/mL
 - 可適靈敏度23.8pg/mL
 - 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
 - 下載英文說明書 中文說明書
 - 規(guī)格96T*596T*1096T*2096T*5096T*100
 - 價格¥ 5378¥ 8964¥ 16135¥ 31374¥ 53784
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特異性
試劑盒中的抗體對三葉因子3(TFF3)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) | 
| APB656Hu01 | 三葉因子3(TFF3)活性蛋白 | Cell?culture;?Activity?Assays. | 
| RPB656Hu01 | 三葉因子3(TFF3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| RPB656Hu02 | 三葉因子3(TFF3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| PAB656Hu02 | 三葉因子3(TFF3)多克隆抗體 | WB; ICC/IF | 
| PAB656Hu01 | 三葉因子3(TFF3)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA. | 
| LAB656Hu71 | 三葉因子3(TFF3)多克隆抗體(生物素標記) | WB; IHC; ICC. | 
| MAB656Hu21 | 三葉因子3(TFF3)單克隆抗體 | WB; IHC; ICC; IP. | 
| MAB656Hu22 | 三葉因子3(TFF3)單克隆抗體 | WB; IHC; ICC; IP. | 
| HEB656Hu | 三葉因子3(TFF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| SEB656Hu | 三葉因子3(TFF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| SCB656Hu | 三葉因子3(TFF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. | 
| LMB656Hu | 三葉因子3(TFF3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| KSB656Hu01 | 三葉因子3(TFF3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". | 
參考文獻
| 雜志 | 參考文獻 | 
| BMC Gastroenterology | Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China[Biomedcentral: Source] | 
| Technology in Cancer Research & Treatment | Serum?Trefoil?Factor?3?as a?Protein?Biomarker?for the?Diagnosis?of?Colorectal?Cancer.[pubmed:27760866] | 
| PLoS One | Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition.[pubmed:29028798] | 
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets.[pubmed:28757275] | 
| Scientific Reports | Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning process[Pubmed:29555969] | 
| Scientific Reports | Kidney-based in vivo model for drug-induced nephrotoxicity testing[Pubmed: 32796873] | 
| International Journal of Molecular Sciences | Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid?…[] | 
| Int. J. Mol. Sci. | Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨1ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und??-[] | 


