神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 編號KSA068Mu01
 - 物種Mus musculus (Mouse,小鼠)相同的名稱,不同的物種。
 - 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
 - 可檢測樣本Serum, plasma, tissue homogenates, cerebrospinal fluid, cell lysates, cell culture supernates and other biological fluids.
 - 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
 - 可檢測范圍7.8-500pg/mL
 - 可適靈敏度3.1pg/mL
 - 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
 - 下載英文說明書 中文說明書
 - 規(guī)格96T*596T*1096T*2096T*5096T*100
 - 價格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
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特異性
試劑盒中的抗體對神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
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