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細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Cytochrome P450 2E1 (CYP2E1)

CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1

  • 細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)細(xì)胞色素P450家族成員2E1(CYP2E1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
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KSA988Mu01細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
BMC Complementary and Alternative MedicineHepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats[PubMed: PMC3605171]
Evidence-Based Complementary Alternative MedicineMechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats[PubMed: PMC3749608]
BMC Complementary and Alternative MedicineCCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat[PubMed: PMC3519517]
Asian Journal of Biochemical and Pharmaceutical ResearchHepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source]
The Scientific World JournalPass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats[Pubmed:24701154]
Journal of Functional FoodsProtective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo[publication:307092820]
CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLAHaloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats.[59f7c85aaca272607e2d8d7a]
Environment InternationalToxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605]
NutrientsOxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages[Pubmed: 31935882]
AntioxidantsEllagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress[34573017]