補體因子D(CFD)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Complement Factor D (CFD)
CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
- 編號KSB833Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.47-30ng/mL
- 可適靈敏度0.22ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對補體因子D(CFD)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB833Mu01 | 補體因子D(CFD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB833Mu02 | 補體因子D(CFD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB833Mu02 | 補體因子D(CFD)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB833Mu01 | 補體因子D(CFD)多克隆抗體 | WB |
| LAB833Mu81 | 補體因子D(CFD)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SEB833Mu | 補體因子D(CFD)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB833Mu | 補體因子D(CFD)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB833Mu01 | 補體因子D(CFD)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of?Diabetes?Investigation | Relationship between serum adipsin and the first phase of glucose‐stimulated insulin secretion in individuals with different glucose tolerance[Pubmed:29432659] |
| Endocrine?Practice | CORRELATION OF INCREASED SERUM ADIPSIN WITH INCREASED CARDIOVASCULAR RISKS IN ADULT PATIENTS WITH GROWTH HORMONE DEFICIENCY[Pubmed: 30657359] |
| BMC Endocrine Disorders | Association between plasma adipsin level and mild cognitive impairment in Chinese patients with type 2 diabetes: a cross-sectional study[Pubmed: 31651303] |
| Scientific Reports | HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse[Pubmed: 33067533] |
| Biomedicine & Pharmacotherapy | Body fat distribution and circulating adipsin are related to metabolic risks in adult patients with newly diagnosed growth hormone deficiency and improve after?…[Pubmed: 33254428] |
| Nephrol Dial Transplant | Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis[33367879] |