胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Pepsin (PP) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA632Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍0.2-200ng/mL
- 靈敏度最小可檢測劑量小于等于0.067 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 89-97 | 93 |
| heparin plasma(n=5) | 95-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-102% | 91-103% | 87-95% | 93-102% |
| EDTA plasma(n=5) | 91-98% | 96-105% | 95-102% | 86-93% |
| heparin plasma(n=5) | 96-104% | 83-101% | 83-94% | 78-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA632Ra | 胃蛋白酶(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA632Ra | 胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA632Ra11 | 胃蛋白酶(PP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Radiology and Oncology | LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMA[Szd:Source] |
| Radiology and Oncology | Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer[Pubmed:Pmc4362607] |
| Clin Otolaryngol | Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative study[PubMed: 25516364] |
| journal of pediatric gastroenterology and nutrition | Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants?[PubMed: 25313850] |
| Journal of Voice | Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia[pubmed:28756936] |
| Sensors (Basel) | Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode[Pubmed:29882917] |
| The Tohoku Journal of Experimental Medicine | Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease[] |
| Respiratory?Research | Pulmonary aspiration in preschool children with cystic fibrosis[Pubmed: 30558606] |
| Tanaffos | Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease Exacerbation[Pubmed: 31423141] |
| Sensors | Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux[Pubmed: 31935973] |
| BIOSCIENCE REPORTS | Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasms[Pubmed: 33103719] |
| OTOLARYNGOLOGY-HEAD AND NECK SURGERY | Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia[Pubmed: 32692278] |
| American Journal of Otolaryngology | Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps[Pubmed: 32889371] |
| The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients[] | |
| Med Sci Monit | The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour??-[33220027] |
| Annals of Esophagus | Pepsin properties, structure, and its accurate measurement: a narrative review[] |
| Laryngoscope | The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis[34338331] |
| Pulmonology | Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia[Pubmed:35715334] |
| Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome[Pubmed:35088760] |