腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)
Instant ELISA Kit for Epinephrine (EPI)
Adrenaline
特異性
                        本試劑盒用于檢測腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型),經檢測與其它相似物質無明顯交叉反應。
                        由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
                    
回收率
分別于定值血清及血漿樣本中加入一定量的腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) | 
| serum(n=5) | 90-97 | 93 | 
| EDTA plasma(n=5) | 84-93 | 89 | 
| heparin plasma(n=5) | 87-101 | 96 | 
精密度
                    精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100 
                    批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
                    批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
                    批內差: CV<10% 
                    批間差: CV<12% 
線性
在定值血清及血漿樣本內加入適量的腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 | 
| serum(n=5) | 86-101% | 80-95% | 84-104% | 87-94% | 
| EDTA plasma(n=5) | 78-103% | 79-90% | 93-101% | 79-95% | 
| heparin plasma(n=5) | 99-105% | 78-98% | 86-94% | 99-105% | 
穩(wěn)定性
                    經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
                    為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
                
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
                            2. 加樣(標準品及樣本)100µL,37°C溫育30分鐘;
                            3. 甩干,加檢測溶液A100µL,37°C溫育30分鐘;
                            4. 洗板3次;
                            5. 加檢測溶液B100µL, 37°C孵育10分鐘;
                            6. 洗板5次;
                            7. 加TMB底物90µL,37°C孵育10-20分鐘;
                            8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)單克隆抗體包被于96孔微孔板中,制成固相載體,向微孔中加入標準品或標本以及同時加入HRP標記腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)的多克隆抗體,孵育結束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
相關產品
| 編號 | 適用物種:Pan-species (General,通用) | 應用(僅供研究使用,不用于臨床診斷!) | 
| CPA858Ge11 | 腎上腺素(EPI)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. | 
| CPA858Ge21 | 腎上腺素(EPI)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. | 
| PAA858Ge01 | 腎上腺素(EPI)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). | 
| MAA858Ge26 | 腎上腺素(EPI)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). | 
| MAA858Ge25 | 腎上腺素(EPI)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). | 
| MAA858Ge22 | 腎上腺素(EPI)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). | 
| CEA858Ge | 腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| IEA858Ge | 腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| HEA858Ge | 腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| MEA858Ge | 腎上腺素(EPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| LMA858Ge | 腎上腺素(EPI)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
| KSA858Ge11 | 腎上腺素(EPI)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". | 
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