Ⅱ型膠原(COL2)真核蛋白
Eukaryotic Collagen Type II (COL2)
- 編號EPA572Rb61
 - 物種Oryctolagus cuniculus (Rabbit,兔)相同的名稱,不同的物種。
 - 來源真核表達(dá)
 - 宿主293F Cell
 - 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
 - 亞細(xì)胞定位分泌, 細(xì)胞外基質(zhì)
 - 預(yù)測分子量27.81kDa
 - 實際分子量33kDa(差異分析請參閱說明書)
 - 片段與標(biāo)簽Ala1253~Leu1487 with N-terminal His Tag
 - 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
 - 性狀凍干粉
 - 純度> 95%
 - 等電點6.7
 - 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載英文說明書 中文說明書
 - 規(guī)格10μg50μg200μg1mg5mg
 - 價格暫無報價暫無報價暫無報價暫無報價暫無報價
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序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種:Oryctolagus cuniculus (Rabbit,兔) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) | 
| EPA572Rb61 | Ⅱ型膠原(COL2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| EPA572Rb62 | Ⅱ型膠原(COL2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| PAA572Rb51 | Ⅱ型膠原(COL2)多克隆抗體 | WB | 
| LAA572Rb71 | Ⅱ型膠原(COL2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. | 
| SEA572Rb | Ⅱ型膠原(COL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| LMA572Rb | Ⅱ型膠原(COL2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. | 
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) | 
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis[PubMed: 22797069] | 
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells[Rsmjournals: Source] | 
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] | 
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly[Pubmed: 24739658] | 
| Spine (Phila Pa 1976). | No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes.[Pubmed:25856264] | 
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] | 
| Materials Science and Engineering: C | Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition[Pubmed:24433895] | 
| 義守大學(xué)生物技術(shù)與化學(xué)工程研究所學(xué)位論文 | 可拋棄式退化性關(guān)節(jié)炎電化學(xué)式尿液免疫感測器之研究 Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis [Publication: U0074-2807201500521300] | 
| Biomed Rep | Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs[PubMed: 26623021] | 
| Macromolecular Bioscience | Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration[PubMed: 25787871] | 
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis[PubMed: 26578310] | 
| Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes[Pubmed:27090063] | 
| 中國眼耳鼻喉科雜志 | 白細(xì)胞介素10 上調(diào)表達(dá)對家兔慢性細(xì)菌性鼻竇炎黏膜創(chuàng)傷修復(fù)的影響[article:11593] | 
| Biomedical Materials | An?innovative?co-axial?system?to?electrospin?in?situ?crosslinked?gelatin?nanofibers.[Pubmed:26987305] | 
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds[pubmed:27821864] | 
| PLoS One. | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.[pubmed:28207788] | 
| Polymers | Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering[2073-4360:8] | 
| PLOS ONE | Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders[10.1371:journal.pone.0187564] | 
| PLoS One | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders[10.1371:journal.pone.0172181] | 
| Platelets. | Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate.[pubmed:29090639] | 
| Molecular?Medicine?Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits[Pubmed:29257344] | 
| Journal of Materials Science | Scaffolds modified with graphene as future implants for nasal cartilage[] | 
| Processes | A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation[] | 



