乳酸脫氫酶(LDH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Lactate Dehydrogenase (LDH)
- 編號(hào)SEB864Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍7.8-500ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于3.1ng/mL.
- 樣本類型serum, plasma, tissue homogenatesand other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價(jià)格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)乳酸脫氫酶(LDH)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的乳酸脫氫酶(LDH)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 85-94 | 90 |
| heparin plasma(n=5) | 92-101 | 95 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的乳酸脫氫酶(LDH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中乳酸脫氫酶(LDH)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 79-96% | 90-97% | 85-101% |
| EDTA plasma(n=5) | 97-105% | 95-102% | 95-103% | 78-104% |
| heparin plasma(n=5) | 89-103% | 99-105% | 91-99% | 79-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將乳酸脫氫酶(LDH)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的乳酸脫氫酶(LDH)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的乳酸脫氫酶(LDH)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的乳酸脫氫酶(LDH)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| PAB864Mu01 | 乳酸脫氫酶(LDH)多克隆抗體 | WB; IHC; ICC; IP. |
| MEB864Mu | 乳酸脫氫酶(LDH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB864Mu | 乳酸脫氫酶(LDH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB864Mu | 乳酸脫氫酶(LDH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neuroscience Research | Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells[PubMed: 20674619] |
| Clinical and Experimental Pharmacology and Physiology | Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity[PubMed: 21261675] |
| Neurochemistry International | Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential[ScienceDirect: S0197018612001428] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients.[Pubmed: 24095588] |
| Journal of Diabetes and its Complications | Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor[Pubmed:25650182] |
| Cancer Science & Therapy | An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice[Open-Access: 1000375] |
| Journal of Antimicrobial Chemotherapy | NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection[PubMed: 26503666] |
| American Journal of Physology Heart and Circulatory Physiology | Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice[Pubmed:27288439] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules[Pubmed:27172385] |
| Experimental Cell Research | UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway[science:S0014482716301598] |
| Blood Cells Mol Dis | Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure[Pubmed:27282581] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice.[pubmed:27907155] |
| Scientific Reports | Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway.[pubmed:27678302] |
| Experimental Biology and Medicine | Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease[pubmed:28534431] |
| Cancer?Science | A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme?…[Scholarena:Source] |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion[Pubmed:29393473] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats[Pubmed:29956781] |
| Oncology?Letters | A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell?dendritic cell?based vaccine[Pubmed:29928442] |
| Experimental Allergy and Immunology | Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation[Pubmed: 30362569] |
| Scientific?Reports | Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis[Pubmed: 30510258] |
| Molecular Medicine Reports | Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury[Pubmed: 32705277] |
| Cell Transplant | Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting?…[Pubmed: 32864999] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles[Pubmed: 32340313] |
| Journal of Renal Injury Prevention | Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction[] |
| clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin?\medicated activation of the FAK?\STAT3 signaling pathway in acute pancreatitis[33634976] |
| Biomed Res Int | Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus[34840966] |
| Cell Cycle | Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells[Pubmed:35316162] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating?…[Pubmed:35281603] |
| Int Immunopharmacol | LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis[Pubmed:35217336] |
